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Addgene

U6-sgRNA-pcr4
(Plasmid #193662)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193662 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    U6-PUM1-sgRNA-GHR-sgRNA-HMGA2-sgRNA-PUM2-sgRNA-tRNA
  • gRNA/shRNA sequence
    GGAACCCAGCAGCCTCAGCCCCA; GTAGAGTCCAGTTGAGGCCAATG; AAGCAGCTCAAAAGGTGAGATTT; ACTACAATTCCC12AGCAGCAGGTA
  • Species
    Synthetic
  • Promoter U6

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    U6-sgRNA-pcr4 was a gift from Liangxue Lai (Addgene plasmid # 193662 ; http://n2t.net/addgene:193662 ; RRID:Addgene_193662)

  • For your References section:

    Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors. Chen F, Lian M, Ma B, Gou S, Luo X, Yang K, Shi H, Xie J, Ge W, Ouyang Z, Lai C, Li N, Zhang Q, Jin Q, Liang Y, Chen T, Wang J, Zhao X, Li L, Yu M, Ye Y, Wang K, Wu H, Lai L. Commun Biol. 2022 Nov 2;5(1):1163. doi: 10.1038/s42003-022-04152-8. 10.1038/s42003-022-04152-8 PubMed 36323848
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