pCMV-RVR-ABE8e-V106W
(Plasmid
#193659)
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PurposeExpression of base editor RVR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 193659 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRVR-dLbCas12a-TadA*8e-V106W, EGFP
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SpeciesSynthetic
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MutationdLbCas12a-G532R/K538V/Y542R, TadA*8e-V106W
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-RVR-ABE8e-V106W was a gift from Liangxue Lai (Addgene plasmid # 193659 ; http://n2t.net/addgene:193659 ; RRID:Addgene_193659) -
For your References section:
Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors. Chen F, Lian M, Ma B, Gou S, Luo X, Yang K, Shi H, Xie J, Ge W, Ouyang Z, Lai C, Li N, Zhang Q, Jin Q, Liang Y, Chen T, Wang J, Zhao X, Li L, Yu M, Ye Y, Wang K, Wu H, Lai L. Commun Biol. 2022 Nov 2;5(1):1163. doi: 10.1038/s42003-022-04152-8. 10.1038/s42003-022-04152-8 PubMed 36323848