pEXP5-NT/pLas eGFP
(Plasmid
#193630)
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PurposeInducible expression of eGFP by the transcription factor complex LasR-3OC12 homoserine lactone (HSL).
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 193630 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEXP5-NT
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2810
- Total vector size (bp) 3669
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Modifications to backbonePromoter is switched from T7 RNAP promoter to Las promoter.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeGFP
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SpeciesSynthetic
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Insert Size (bp)861
- Promoter T7 RNAP promoter
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TCGGTGATTCATTCTGCT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.03.04.531082v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEXP5-NT/pLas eGFP was a gift from Dora Tang (Addgene plasmid # 193630 ; http://n2t.net/addgene:193630 ; RRID:Addgene_193630) -
For your References section:
Bidirectional communication between droplet interface bilayers driven by cell-free quorum sensing gene circuits. Gonzales DT, Suraritdechachai S, Zechner C, Tang T-YD. ChemSystemsChem 2023 10.1002/syst.202300029
Map uploaded by the depositor.
