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Addgene

pAG300
(Plasmid #19363)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19363 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVT102U
  • Backbone manufacturer
    Vernet et al 1987
  • Backbone size (bp) 6400
  • Modifications to backbone
    SfiIA (GGCCATTACGGCC) and SfiIB (GGCCGCCTCGGCC) sites added for unidirectional cloning.
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG300 was a gift from Holger Deising (Addgene plasmid # 19363 ; http://n2t.net/addgene:19363 ; RRID:Addgene_19363)

  • For your References section:

    Sfp-type 4'-phosphopantetheinyl transferase is indispensable for fungal pathogenicity. Horbach R, Graf A, Weihmann F, Antelo L, Mathea S, Liermann JC, Opatz T, Thines E, Aguirre J, Deising HB. Plant Cell. 2009 Oct;21(10):3379-96. doi: 10.1105/tpc.108.064188. Epub 2009 Oct 30. 10.1105/tpc.108.064188 PubMed 19880801
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