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Addgene

pEXP5-NT/pLux LuxI
(Plasmid #193627)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193627 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEXP5-NT
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2775
  • Total vector size (bp) 3453
  • Modifications to backbone
    Promoter is switched from T7 RNAP promoter to Lux promoter.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LuxI
  • Species
    Synthetic; Vibrio fischeri
  • Insert Size (bp)
    678
  • Promoter Lux promoter
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEXP5-NT/pLux LuxI was a gift from Dora Tang (Addgene plasmid # 193627 ; http://n2t.net/addgene:193627 ; RRID:Addgene_193627)

  • For your References section:

    Bidirectional communication between droplet interface bilayers driven by cell-free quorum sensing gene circuits. Gonzales DT, Suraritdechachai S, Zechner C, Tang T-YD. ChemSystemsChem 2023 10.1002/syst.202300029
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