pDL026_HA-rb_IgG_LC_15F11
(Plasmid #193623)

• Purpose: HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.
• Depositing Lab: Jennifer DeLuca
• Publication: DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi: 10.7554/eLife.72093.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: clontech
• Backbone size w/o insert (bp): 3952
• Total vector size (bp): 4674
• Modifications to backbone: pEGFP was digested with Sac1 and Not1 during cloning to remove the GFP tag. The Not1 restriction site was also mutated.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Anti-HA_15F11 rabbit light chain
• Species: Synthetic
• Insert Size (bp): 710
• Promoter: CMV
• Tag / Fusion Protein:
  • signal peptide (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-For
• 3′ sequencing primer: SV40pA-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA