pDL002_Hec1-ms_IgG_LC
(Plasmid #193619)

• Purpose: Hec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL001 in HEK293 cells.
• Depositing Lab: Jennifer DeLuca
• Publication: DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi: 10.7554/eLife.72093.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: clontech
• Backbone size w/o insert (bp): 3952
• Total vector size (bp): 4681
• Modifications to backbone: pEGFP-N1 was digested with Sac1 and Not1 to remove GFP.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Anti-Hec1 light chain (Hec1-ms_IgG_LC)
• Species: M. musculus (mouse)
• Insert Size (bp): 720
• Promoter: CMV
• Tag / Fusion Protein:
  • signal peptide (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-For
• 3′ sequencing primer: SV40pA-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA