pDL001_Hec1-ms_IgG_HC
(Plasmid #193618)

• Purpose: Hec1 heavy chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL002 in HEK293 cells.
• Depositing Lab: Jennifer DeLuca
• Publication: DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi: 10.7554/eLife.72093.

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 3952
• Total vector size (bp): 5392
• Modifications to backbone: pEGFP-N1 was digested with Sac1/Not1 to remove GFP.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Anti-Hec1 Heavy Chain (Hec1-ms_IgG_HC)
• Species: M. musculus (mouse)
• Insert Size (bp): 1431
• Promoter: CMV
• Tags / Fusion Proteins:
  • His6 (C terminal on insert)
  • signal peptide (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-For
• 3′ sequencing primer: SV40pA-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA