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Addgene

3xFLAG-FKBP-LRRK2
(Plasmid #193584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193584 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST
  • Backbone size w/o insert (bp) 5138
  • Total vector size (bp) 12722
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Leucine-rich repeat kinase 2
  • Alt name
    LRRK2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7584
  • Entrez Gene
    LRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG and FKBP (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ATGGCTAGTGGCAGCTGTCAGGGGTGCGAAGAGGACGAGGAAACTCTGAAG
  • 3′ sequencing primer TTACTCAACAGATGTTCGTCTCATTTTT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3xFLAG-FKBP-LRRK2 was a gift from Mark Cookson (Addgene plasmid # 193584 ; http://n2t.net/addgene:193584 ; RRID:Addgene_193584)

  • For your References section:

    Lysosomal positioning regulates Rab10 phosphorylation at LRRK2(+) lysosomes. Kluss JH, Beilina A, Williamson CD, Lewis PA, Cookson MR, Bonet-Ponce L. Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2205492119. doi: 10.1073/pnas.2205492119. Epub 2022 Oct 18. 10.1073/pnas.2205492119 PubMed 36256825

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