LYSO-LRRK2
(Plasmid #193582)

• Purpose: Expresses human full-length LRRK2 with a lysosomal targeting sequence from LAMTOR1 (1-39aa) and 3xFLAG on the N-terminus
• Depositing Lab: Mark Cookson
• Publication: Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2205492119. doi: 10.1073/pnas.2205492119. Epub 2022 Oct 18.

Backbone

• Vector backbone: pDEST
• Backbone size w/o insert (bp): 5060
• Total vector size (bp): 12512
• Modifications to backbone: LAMTOR1(1-39aa) and 3xFLAG sequences
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Leucine-rich repeat kinase 2
• Alt name: LRRK2
• Species: H. sapiens (human)
• Insert Size (bp): 7584
• Entrez Gene: LRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
• Promoter: CMV
• Tag / Fusion Protein:
  • LAMTOR1(1-39aa) and 3xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: ATGGCTAGTGGCAGCTGTCAGGGGTGCGAAGAGGACGAGGAAACTCTGAAG
• 3′ sequencing primer: AAAAATGAGACGAACATCTGTTGAGTAA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry