UAS-Cre-T2A-miRFP670
(Plasmid #193581)

• Purpose: Fluorescent reporter for Gal4-induced Cre recombination
• Depositing Lab: Satoru Takahashi
• Publication: Yoshihara et al Exp Anim. 2022 Aug 5;71(3):385-390. doi: 10.1538/expanim.21-0202. Epub 2022 Apr 21.

Backbone

• Vector backbone: pUAS-Cre
• Backbone manufacturer: Connie Cepko's lab
• Backbone size w/o insert (bp): 4036
• Total vector size (bp): 5128
• Modifications to backbone: SV40 poly(A) signal was replaced with T2A-miRFP670 and bGH poly(A) signal
• Vector type: Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: miRFP670
• Alt name: iRFP670
• Species: Rhodopseudomonas palustris
• Insert Size (bp): 948
• GenBank ID: KX421097.1
• Promoter: 5X UAS
• Tag / Fusion Protein:
  • T2A (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: FseI (destroyed during cloning)
• 3′ cloning site: HpaI (destroyed during cloning)
• 5′ sequencing primer: AGAATTCGGCAGTGGAGAGGG
• 3′ sequencing primer: AAAAGGTACCTCCCCAGCATGCCTGCTATT

Resource Information

• Supplemental Documents:
  • Supplementary_Data_2.ape
• A portion of this plasmid was derived from a plasmid made by: pSpCas9 (BB)-2AmiRFP670 was a gift from Ralf Kuehn (Addgene plasmid #91854; http://n2t.net/addgene:91854 ; RRID: Addgene_91854).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry