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Addgene

12.4kbVillin-ΔATG
(Plasmid #19358)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19358 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    puc18
  • Backbone size w/o insert (bp) 2686
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    12.4kb Villin promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    14000
  • Mutation
    No ATG - transgenes cloned downstream of the villin promoter must include an ATG.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13 Reverse
  • 3′ sequencing primer M13 Forward
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is an updated version of the pBSII-12.4kbVillin promoter used in the cited publication. This vector is optimized with a larger, more useful MCS and easy linearization for transgenic injection. 12.4kb villin is a promoter to drive intestinal-specific expression of transgenes in the mouse intestinal epithelium. This construct was created by addition of two polylinker oligos between the HindIII and EcoR1 sites (replacing the MCS) in the puc18 vector, and then addition of the 12.4kb villin promoter (between EcoR1 and Sma1) and an SV40Pa (between HindIII and EcoRV) of the resulting MCS. Unique sites between the 12.4kb Villin promoter and SV40Pa are (from 5' to 3'): Sma1, Xho1, Mlu1, Cla1, SacII, Age1, Kpn1. Pre-transgene injection linearization of the plasmid can be obtained by digestion with PmeI, which removes the puc18 sequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    12.4kbVillin-ΔATG was a gift from Deborah Gumucio (Addgene plasmid # 19358 ; http://n2t.net/addgene:19358 ; RRID:Addgene_19358)
  • For your References section:

    Cis elements of the villin gene control expression in restricted domains of the vertical (crypt) and horizontal (duodenum, cecum) axes of the intestine. Madison BB, Dunbar L, Qiao XT, Braunstein K, Braunstein E, Gumucio DL. J Biol Chem. 2002 Sep 6. 277(36):33275-83. 10.1074/jbc.M204935200 PubMed 12065599