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Addgene

PH-miRFP-FRBx2
(Plasmid #193569)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193569 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 6039
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PH domain from PLC delta1
  • Alt name
    Plcd1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    407
  • Mutation
    PH domain
  • Entrez Gene
    Plcd1 (a.k.a. Plc1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • miRFP703 (C terminal on insert)
    • FRB (T2098L) (C terminal on insert)
    • FRB (T2098L) (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Dr. T. Inoue (Johns Hopkins University) and addgene #79997

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.03.21.485138 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PH-miRFP-FRBx2 was a gift from Justin Taraska (Addgene plasmid # 193569 ; http://n2t.net/addgene:193569 ; RRID:Addgene_193569)

  • For your References section:

    A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells. Obashi K, Sochacki KA, Strub MP, Taraska JW. Nat Commun. 2023 Feb 9;14(1):732. doi: 10.1038/s41467-023-36304-7. 10.1038/s41467-023-36304-7 PubMed 36759616
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