pIM61
(Plasmid
#193543)
-
PurposepENTRY clone for NLR Integrated Domain ID#57
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 193543 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepTWIST ENTRY
-
Vector typepENTRY clone
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameID#57
-
SpeciesBrachypodium (Bradi4g09648.1.p)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pIM61 was a gift from Nemo Peeters (Addgene plasmid # 193543 ; http://n2t.net/addgene:193543 ; RRID:Addgene_193543) -
For your References section:
An NLR integrated domain toolkit to identify plant pathogen effector targets. Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, Dabos P, Deslandes L, Peeters N. Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 2023 Jul 24. 10.1111/tpj.16331 PubMed 37248633