pFA6a-bleMX6-P81nmt1-3FLAG
(Plasmid
#19353)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19353 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a-kanMX6-P81nmt1-3FLAG
- Backbone size w/o insert (bp) 4000
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Vector typeYeast Expression
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Selectable markersPhleomycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5 alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namebleMX6
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SpeciesStreptoalloteichus hindustanus
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Insert Size (bp)1000
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site PmeI (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-bleMX6-P81nmt1-3FLAG was a gift from Eishi Noguchi (Addgene plasmid # 19353 ; http://n2t.net/addgene:19353 ; RRID:Addgene_19353) -
For your References section:
A vector system for genomic FLAG epitope-tagging in Schizosaccharomyces pombe. Noguchi C, Garabedian MV, Malik M, Noguchi E. Biotechnol J. 2008 Aug 26. ():. 10.1002/biot.200800140 PubMed 18729046
Map uploaded by the depositor.
