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pKPI-MtLAP1
(Plasmid #193521)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193521 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pL2V-1-50507
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    MtLAP1
  • Alt name
    Legume Anthocyanin production 1
  • Species
    Medicago truncatula
  • GenBank ID
    FJ199998
  • Promoter Medtr8g059790(KPI)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    DsRed
  • Alt name
    Red fluorescent protein
  • Species
    Discosoma sp
  • GenBank ID
    AAU04444
  • Promoter ATUBI10

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKPI-MtLAP1 was a gift from Jeremy Murray (Addgene plasmid # 193521 ; http://n2t.net/addgene:193521 ; RRID:Addgene_193521)

  • For your References section:

    Anthocyanin pigmentation as a quantitative visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots. Kumar A, Lin H, Li Q, Ruan Y, Cousins D, Li F, Gao S, Jackson K, Wen J, Murray JD, Xu P. New Phytol. 2022 Sep 20. doi: 10.1111/nph.18504. 10.1111/nph.18504 PubMed 36128658
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