pL1-pKPI-MtLAP1-Adh
(Plasmid
#193520)
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PurposeAnthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 193520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepL1V-F2-47742
- Backbone size w/o insert (bp) 4968
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMtLAP1
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Alt nameLegume Anthocyanin production 1
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SpeciesMedicago truncatula
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GenBank IDFJ199998
- Promoter Medtr8g059790(KPI)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pL1-pKPI-MtLAP1-Adh was a gift from Jeremy Murray (Addgene plasmid # 193520 ; http://n2t.net/addgene:193520 ; RRID:Addgene_193520) -
For your References section:
Anthocyanin pigmentation as a quantitative visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots. Kumar A, Lin H, Li Q, Ruan Y, Cousins D, Li F, Gao S, Jackson K, Wen J, Murray JD, Xu P. New Phytol. 2022 Sep 20. doi: 10.1111/nph.18504. 10.1111/nph.18504 PubMed 36128658