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Addgene

ChlorON-2
(Plasmid #193478)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193478 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1(+)-N-6His
  • Backbone manufacturer
    Genscript
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ChlorON-2
  • Species
    Synthetic
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ChlorON-2 was a gift from Sheel Dodani (Addgene plasmid # 193478 ; http://n2t.net/addgene:193478 ; RRID:Addgene_193478)

  • For your References section:

    Engineering the ChlorON Series: Turn-On Fluorescent Protein Sensors for Imaging Labile Chloride in Living Cells. Tutol JN, Ong WSY, Phelps SM, Peng W, Goenawan H, Dodani SC. ACS Cent Sci. 2023 Dec 18;10(1):77-86. doi: 10.1021/acscentsci.3c01088. eCollection 2024 Jan 24. 10.1021/acscentsci.3c01088 PubMed 38292617
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