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PurposePromoterless mcherry donor for Pa01
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 193466 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCB214
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePa01_attP
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Speciessourced from various phage and bacterial genomes, human codon-optomized
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAF0687 Pa01_attP-mCherry donor was a gift from Patrick Hsu (Addgene plasmid # 193466 ; http://n2t.net/addgene:193466 ; RRID:Addgene_193466) -
For your References section:
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome. Durrant MG, Fanton A, Tycko J, Hinks M, Chandrasekaran SS, Perry NT, Schaepe J, Du PP, Lotfy P, Bassik MC, Bintu L, Bhatt AS, Hsu PD. Nat Biotechnol. 2022 Oct 10. pii: 10.1038/s41587-022-01494-w. doi: 10.1038/s41587-022-01494-w. 10.1038/s41587-022-01494-w PubMed 36217031