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Addgene

pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry
(Plasmid #193465)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193465 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCB250
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dn29_attP
  • Species
    sourced from various phage and bacterial genomes, human codon-optomized
  • Promoter Ef1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry was a gift from Patrick Hsu (Addgene plasmid # 193465 ; http://n2t.net/addgene:193465 ; RRID:Addgene_193465)

  • For your References section:

    Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome. Durrant MG, Fanton A, Tycko J, Hinks M, Chandrasekaran SS, Perry NT, Schaepe J, Du PP, Lotfy P, Bassik MC, Bintu L, Bhatt AS, Hsu PD. Nat Biotechnol. 2022 Oct 10. pii: 10.1038/s41587-022-01494-w. doi: 10.1038/s41587-022-01494-w. 10.1038/s41587-022-01494-w PubMed 36217031
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