pFA6a-natMX6-P41nmt1-3FLAG
(Plasmid
#19346)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 19346 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6a-kanMX6-P41nmt1-3FLAG
- Backbone size w/o insert (bp) 4000
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Vector typeYeast Expression
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Selectable markersnourseothricin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenatMX6
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SpeciesStreptomyces noursei
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Insert Size (bp)1200
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site PmeI (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There are a few point mutations in drug resistant genes. These mutations are derived from the original drug resistant plasmids we have obtained. However these mutations do not alter drug resistant property of the genes or the functions of our plasmids.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-natMX6-P41nmt1-3FLAG was a gift from Eishi Noguchi (Addgene plasmid # 19346 ; http://n2t.net/addgene:19346 ; RRID:Addgene_19346) -
For your References section:
A vector system for genomic FLAG epitope-tagging in Schizosaccharomyces pombe. Noguchi C, Garabedian MV, Malik M, Noguchi E. Biotechnol J. 2008 Aug 26. ():. 10.1002/biot.200800140 PubMed 18729046