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Addgene

vHB178
(Plasmid #193457)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193457 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    vHB66
  • Vector type
    Pandoravirus/mollivirus Expression
  • Selectable markers
    nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    3xHA
  • Species
    Synthetic
  • Promoter ----

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTTGTTGCGCGAATTcTACCCATACGATGTTCCTGAC
  • 3′ sequencing primer aaaatAAGAACAAGAGTTAAGCGTAATCTGGAACGTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    vHB178 was a gift from Chantal Abergel (Addgene plasmid # 193457 ; http://n2t.net/addgene:193457 ; RRID:Addgene_193457)

  • For your References section:

    Evolution of giant pandoravirus revealed by CRISPR/Cas9. Bisio H, Legendre M, Giry C, Philippe N, Alempic JM, Jeudy S, Abergel C. Nat Commun. 2023 Jan 26;14(1):428. doi: 10.1038/s41467-023-36145-4. 10.1038/s41467-023-36145-4 PubMed 36702819
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