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Addgene

vHB8
(Plasmid #193454)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193454 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    vc2
  • Backbone size w/o insert (bp) 5699
  • Total vector size (bp) 10526
  • Vector type
    CRISPR
  • Selectable markers
    geneticin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP-Cas9-NLS
  • gRNA/shRNA sequence
    NotI restriction site
  • Species
    Synthetic; Acanthamoeba castellanii
  • Promoter GAPDH
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACCAATCCACCCATATGGTGAGCAAGGGCGAGGAGC
  • 3′ sequencing primer GGTGGGGGCATCTAGATTAGTCACCGCCCAGCTGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    vHB8 was a gift from Chantal Abergel (Addgene plasmid # 193454 ; http://n2t.net/addgene:193454 ; RRID:Addgene_193454)

  • For your References section:

    Evolution of giant pandoravirus revealed by CRISPR/Cas9. Bisio H, Legendre M, Giry C, Philippe N, Alempic JM, Jeudy S, Abergel C. Nat Commun. 2023 Jan 26;14(1):428. doi: 10.1038/s41467-023-36145-4. 10.1038/s41467-023-36145-4 PubMed 36702819
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