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vc241
(Plasmid #193453)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193453 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    vc2
  • Backbone size w/o insert (bp) 675
  • Total vector size (bp) 5580
  • Modifications to backbone
    Geneticin selection cassette has been repleaced for a nourseothricin selection cassette
  • Vector type
    Acanthamoeba exression
  • Selectable markers
    nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mRFP
  • Species
    Acanthamoeba castellanii
  • Insert Size (bp)
    675
  • Promoter GAPDH

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer gccCATATGGCCTCCTCCGAGGACGTC
  • 3′ sequencing primer gcctctagattaGGCGCCGGTGGAGTG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    vc241 was a gift from Chantal Abergel (Addgene plasmid # 193453 ; http://n2t.net/addgene:193453 ; RRID:Addgene_193453)

  • For your References section:

    Evolution of giant pandoravirus revealed by CRISPR/Cas9. Bisio H, Legendre M, Giry C, Philippe N, Alempic JM, Jeudy S, Abergel C. Nat Commun. 2023 Jan 26;14(1):428. doi: 10.1038/s41467-023-36145-4. 10.1038/s41467-023-36145-4 PubMed 36702819
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