pFA6a-5FLAG-bleMX6
(Plasmid #19344)

• Depositing Lab: Eishi Noguchi
• Publication: Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Backbone

• Vector backbone: pFA6a-5FLAG-kanMX6
• Backbone size w/o insert (bp): 2900
• Vector type: Yeast Expression
• Selectable markers: Phleomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5 alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: bleMX6
• Species: Streptoalloteichus hindustanus
• Insert Size (bp): 1000

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: PmeI (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are a few point mutations in drug resistant genes. These mutations are derived from the original drug resistant plasmids we have obtained. However these mutations do not alter drug resistant property of the genes or the functions of our plasmids.