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Addgene

nTALECD for_CYO1 exon2_1397NC
(Plasmid #193377)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193377 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pK7WG2
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    For specific C-to-T base editing to introduce a nonsense mutation in the coding sequence of AtCYO1.
  • Species
    Synthetic

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ti binary vector constructed by multisite Gateway. Please visit https://doi.org/10.1101/2023.02.27.530312 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    nTALECD for_CYO1 exon2_1397NC was a gift from Shin-ichi Arimura (Addgene plasmid # 193377 ; http://n2t.net/addgene:193377 ; RRID:Addgene_193377)

  • For your References section:

    TALE-based C-to-T base editor for multiple homologous genes with flexible precision. Hosoda A, Nakazato I, Takanashi H, Tsutsumi N, Arimura SI. bioRxiv 2023.02.27.530312 10.1101/2023.02.27.530312
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