p4RV
(Plasmid
#193375)
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PurposeContains the fourth TALE repeat harboring RV as RVD
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 193375 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBSK
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Vector typeTALEN
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRepeat 4 RV
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SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Platinum Gate system vector to assemble TALE repeat domain. Please visit https://doi.org/10.1101/2023.02.27.530312 for bioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p4RV was a gift from Shin-ichi Arimura (Addgene plasmid # 193375 ; http://n2t.net/addgene:193375 ; RRID:Addgene_193375) -
For your References section:
TALE-based C-to-T base editor for multiple homologous genes with flexible precision. Hosoda A, Nakazato I, Takanashi H, Tsutsumi N, Arimura SI. bioRxiv 2023.02.27.530312 10.1101/2023.02.27.530312