E2_pENTR_R4-R3_HSPter-RPS5A-NLS
(Plasmid
#193371)
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Purpose(Empty Backbone) nTALEN or nTALECD vector assembly, Entry clone for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 193371 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePINII
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Multisite Gateway entry vector to express ORFs from other entry clone. HSP terminator at the downstream of attR4. RPS5A promoter and SV40 nuclear localization signal at the upstream of attR3. Please visit https://doi.org/10.1101/2023.02.27.530312 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
E2_pENTR_R4-R3_HSPter-RPS5A-NLS was a gift from Shin-ichi Arimura (Addgene plasmid # 193371 ; http://n2t.net/addgene:193371 ; RRID:Addgene_193371) -
For your References section:
TALE-based C-to-T base editor for multiple homologous genes with flexible precision. Hosoda A, Nakazato I, Takanashi H, Tsutsumi N, Arimura SI. bioRxiv 2023.02.27.530312 10.1101/2023.02.27.530312