pETM30-C9ORF78_F8A
(Plasmid #193369)

• Purpose: Expression of C9ORF78 F8A variant in E.coli with N-terminal His6-GST-tag which is cleavable with TEV protease
• Depositing Lab: Markus Wahl
• Publication: Bergfort et al Nat Commun. 2022 Mar 3;13(1):1132. doi: 10.1038/s41467-022-28754-2.

Backbone

• Vector backbone: pETM30
• Backbone manufacturer: EMBL Heidelberg
• Backbone size w/o insert (bp): 6346
• Total vector size (bp): 6860
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: C9ORF78_F8A
• Species: H. sapiens (human)
• Insert Size (bp): 870
• Mutation: F8A, the mutation is located in the binding interface with SNRNP200
• GenBank ID: 51759
• Entrez Gene: C9orf78 (a.k.a. CSU2, HCA59, HSPC220, TLS1, bA409K20.3)
• Promoter: T7
• Tag / Fusion Protein:
  • His6-GST-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GEX5-for (Microsynth)
• 3′ sequencing primer: T7term

Resource Information

• Supplemental Documents:
  • pETM30-C9ORF78_F8A.gb
• A portion of this plasmid was derived from a plasmid made by: C9ORF78 wild type was provided by the laboratory of Prof. Ulrich Stelzel, University of Graz (Austria) in a yeast expression vector.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry