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pETM30-C9ORF78_R41A
(Plasmid #193368)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193368 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETM30
  • Backbone manufacturer
    EMBL Heidelberg
  • Backbone size w/o insert (bp) 6346
  • Total vector size (bp) 6860
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    C9ORF78_R41A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    870
  • Mutation
    R41A, the mutation is located in the binding interface with SNRNP200
  • GenBank ID
    51759
  • Entrez Gene
    C9orf78 (a.k.a. CSU2, HCA59, HSPC220, TLS1, bA409K20.3)
  • Promoter T7
  • Tag / Fusion Protein
    • His6-GST-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GEX5-for (Microsynth)
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    C9ORF78 wild type was provided by the laboratory of Prof. Ulrich Stelzel, University of Graz (Austria) in a yeast expression vector.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETM30-C9ORF78_R41A was a gift from Markus Wahl (Addgene plasmid # 193368 ; http://n2t.net/addgene:193368 ; RRID:Addgene_193368)

  • For your References section:

    A multi-factor trafficking site on the spliceosome remodeling enzyme BRR2 recruits C9ORF78 to regulate alternative splicing. Bergfort A, Preussner M, Kuropka B, Ilik IA, Hilal T, Weber G, Freund C, Aktas T, Heyd F, Wahl MC. Nat Commun. 2022 Mar 3;13(1):1132. doi: 10.1038/s41467-022-28754-2. 10.1038/s41467-022-28754-2 PubMed 35241646
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