pc3.1-CAGGS-ASAP3-mCyRFP3
(Plasmid #193323)

• Purpose: A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3
• Depositing Lab: Michael Lin
• Publication: Kim et al Sci Rep. 2022 Mar 7;12(1):3678. doi: 10.1038/s41598-022-07313-1.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 6103
• Total vector size (bp): 2007
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ASAP3-R3
• Species: G. gallus (chicken); jelly fish, Entacmaea quadricolor
• Insert Size (bp): 2007
• Promoter: caggs

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • Annotated Depositor Map.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the Addgene NGS result contains ambiguous bases in the CAG promoter due to its GC-rich nature. Addgene's maps are automatically annotated by SnapGene. The mCyRFP3 fluorophore may be mis-annotated since not all variants are included on the common list of features. Please see the Annotated Depositor Map in the Supplemental Documents section for reference.