HYG-TET-OFF – ubiquitin – LEU – 4MYC
(Plasmid
#193316)
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PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 193316 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneUnknown
- Total vector size (bp) 5690
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Vector typeBacterial Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUbiquitin - Leucine - 4MYC N-terminal tagging
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SpeciesS. cerevisiae (budding yeast)
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Tag
/ Fusion Protein
- 4MYC (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HYG-TET-OFF – ubiquitin – LEU – 4MYC was a gift from Rob Mitra (Addgene plasmid # 193316 ; http://n2t.net/addgene:193316 ; RRID:Addgene_193316) -
For your References section:
A Suite of New Strain Construction Vectors for Gene Expression Knockdown in Budding Yeast. Shively CA, Dong F, Mitra RD. ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547. 10.1021/acssynbio.2c00547 PubMed 36650116
