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Perox-mTurq2-Apollo-NADP+
(Plasmid #193304)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193304 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G6PD
  • Alt name
    glucose-6-phosphate dehydrogenase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2736
  • Mutation
    S40A, R72Q, H201N, K205T
  • Entrez Gene
    G6PD (a.k.a. G6PD1)
  • Promoter T7
  • Tag / Fusion Protein
    • mTurqoise2, peroxisomal targeting sequence (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (mTurq2) (unknown if destroyed)
  • 3′ cloning site XhoI/XbaI (mTurq2) (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    G6PD cloned from vector purchased from DNASU. Clone ID: FLH023345.01L

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains a C-terminal mTurqoise2 tag. Contains a C-terminal -SKL peroxisomal targeting sequence.

Please visit https://doi.org/10.1101/2022.04.27.489691 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Perox-mTurq2-Apollo-NADP+ was a gift from Jonathan Rocheleau (Addgene plasmid # 193304 ; http://n2t.net/addgene:193304 ; RRID:Addgene_193304)

  • For your References section:

    Targeting Apollo-NADP(+) to Image NADPH Generation in Pancreatic Beta-Cell Organelles. Chang HH, Bennett AM, Cameron WD, Floro E, Au A, McFaul CM, Yip CM, Rocheleau JV. ACS Sens. 2022 Oct 21. doi: 10.1021/acssensors.2c01174. 10.1021/acssensors.2c01174 PubMed 36269889
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