Mito-mTurq2-Apollo-NADP+
(Plasmid #193303)

• Purpose: Expresses mTurq2-Apollo-NADP+ sensor localized to the mitochondria
• Depositing Lab: Jonathan Rocheleau
• Publication: Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acssensors.2c01174.

Backbone

• Vector backbone: pcDNA3.1(+)
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G6PD
• Alt name: glucose-6-phosphate dehydrogenase
• Species: H. sapiens (human)
• Insert Size (bp): 2736
• Mutation: S40A, R72Q, H201N, K205T
• Entrez Gene: G6PD (a.k.a. G6PD1)
• Promoter: T7
• Tags / Fusion Proteins:
  • mitochondrial targeting sequence (N terminal on insert)
  • mTurqoise2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (mTurq2) (unknown if destroyed)
• 3′ cloning site: XhoI/XbaI (mTurq2) (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • Mito-mTurq2-Apollo-NADP+.gbk
• A portion of this plasmid was derived from a plasmid made by: G6PD cloned from vector purchased from DNASU. Clone ID: FLH023345.01L

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains a C-terminal mTurqoise2 tag. Contains an N-terminal tag of 4 copies of Cox8 mitochondrial targeting sequence.

Please visit https://doi.org/10.1101/2022.04.27.489691 for bioRxiv preprint.