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pMF-FU_TetO_GW_PPARG2
(Plasmid #193289)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193289 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FU-tetO-Gateway
  • Backbone manufacturer
    Addgene plasmid 43914
  • Backbone size w/o insert (bp) 10257
  • Total vector size (bp) 9962
  • Modifications to backbone
    Removal of Gateway cassette and insertion of PPARG2 through gateway recombination
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PPARG2
  • Alt name
    NR1C3
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_015869.5
  • Entrez Gene
    PPARG2 (a.k.a. HUMPPARG)
  • Promoter TRE promoter, Tet-ON

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMF-FU_TetO_GW_PPARG2 was a gift from Rudolf Jaenisch (Addgene plasmid # 193289 ; http://n2t.net/addgene:193289 ; RRID:Addgene_193289)

  • For your References section:

    Development of a physiological insulin resistance model in human stem cell-derived adipocytes. Friesen M, Khalil AS, Barrasa MI, Jeppesen JF, Mooney DJ, Jaenisch R. Sci Adv. 2022 Jun 17;8(24):eabn7298. doi: 10.1126/sciadv.abn7298. Epub 2022 Jun 17. 10.1126/sciadv.abn7298 PubMed 35714187
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