pMF-TetTight
(Plasmid #193288)

• Purpose: Lentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgene
• Depositing Lab: Rudolf Jaenisch
• Publication: Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi: 10.1126/sciadv.abn7298. Epub 2022 Jun 17.

Backbone

• Vector backbone: FUdeltaGW
• Backbone manufacturer: Addgene 19780
• Backbone size w/o insert (bp): 9899
• Total vector size (bp): 10817
• Modifications to backbone: Addition of P2A-tTS to expression cassette
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: rtTA-P2A-tTS
• Species: Synthetic
• Insert Size (bp): 1654
• Promoter: EF‐1α

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xcm1 (unknown if destroyed)
• 3′ cloning site: EcoR1 (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • Tet-Tight FUdeltaGW_rtTA_p2A-tTS.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry