mEos3.2-cortactin
(Plasmid
#193287)
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Purposemammalian transient expression of mEos3.2-cortactin
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 193287 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 4902
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecortactin
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SpeciesM. musculus (mouse)
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Entrez GeneCttn (a.k.a. 1110020L01Rik, Ems1)
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Tag
/ Fusion Protein
- mEos3.2 (N terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byAddgene 26722
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEos3.2-cortactin was a gift from Klaus Hahn (Addgene plasmid # 193287 ; http://n2t.net/addgene:193287 ; RRID:Addgene_193287) -
For your References section:
Actin nano-architecture of phagocytic podosomes. Herron JC, Hu S, Watanabe T, Nogueira AT, Liu B, Kern ME, Aaron J, Taylor A, Pablo M, Chew TL, Elston TC, Hahn KM. Nat Commun. 2022 Jul 27;13(1):4363. doi: 10.1038/s41467-022-32038-0. 10.1038/s41467-022-32038-0 PubMed 35896550