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Addgene

CBE-T1.30
(Plasmid #193280)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193280 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMV/pBR322
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CBE-T1.30
  • Species
    Other
  • Mutation
    D10A (Cas9), TadA mutations within
  • Promoter CMV

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CBE-T1.30 was a gift from Nicole Gaudelli (Addgene plasmid # 193280 ; http://n2t.net/addgene:193280 ; RRID:Addgene_193280)

  • For your References section:

    Improved cytosine base editors generated from TadA variants. Lam DK, Feliciano PR, Arif A, Bohnuud T, Fernandez TP, Gehrke JM, Grayson P, Lee KD, Ortega MA, Sawyer C, Schwaegerle ND, Peraro L, Young L, Lee SJ, Ciaramella G, Gaudelli NM. Nat Biotechnol. 2023 Jan 9. doi: 10.1038/s41587-022-01611-9. 10.1038/s41587-022-01611-9 PubMed 36624149
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