pML2003
(Plasmid #193255)

• Purpose: Propionate sensor expressing eYFP reporter
• Depositing Lab: Lauren Andrews
• Publication: Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:938056. doi: 10.3389/fbioe.2022.938056. eCollection 2022.

Backbone

• Vector backbone: pAN1201
• Total vector size (bp): 6884
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: prpR
• Insert Size (bp): 1587
• Promoter: Synthetic promoter P6

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: SapI (destroyed during cloning)
• 3′ cloning site: SapI (destroyed during cloning)
• 5′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: eYFP
• Insert Size (bp): 720
• Promoter: Pprp

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (destroyed during cloning)
• 3′ cloning site: BbsI (destroyed during cloning)
• 5′ sequencing primer: TGGCTCATAACACCCCTTG

Resource Information

• Supplemental Documents:
  • pML2003.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry