pSMT-MjtRNA-GFP(47TAG)
(Plasmid #193251)

• Purpose: Expresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)
• Depositing Labs: Peter Schultz, Jessica Seeliger
• Publication: Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348. doi: 10.1021/acsinfecdis.6b00179. Epub 2017 Mar 21.

Backbone

• Vector backbone: pSMT3
• Vector type: Bacterial Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: GFP(47TAG)
• Alt name: GFP(151TAG), GFP(Tyr151TAG), eGFP
• Mutation: Codon for Tyr47 replaced with amber stop codon TAG (nucleotide position 151).
• Promoter: hsp60

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: CTAGAGGTGACCACAACG

Gene/Insert 2

• Gene/Insert name: MjtRNA
• Alt name: tRNATyr
• Species: M. jannaschii
• Promoter: metU

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: gccgggatcactctcg

Resource Information

• Supplemental Documents:
  • Previti_B7F_1_pSMT-GFP47∆TAG_pLann.gbk
• A portion of this plasmid was derived from a plasmid made by: Received from Peter Schultz (Scripps)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid was originally reported by the Peter Schultz lab in Wang, Schultz et al. PLoS One 2010, doi: 10.1371/journal.pone.0009354. Please see associated publication Ganapathy, Seeliger et al. for full details regarding any additional cloning that was performed.