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Addgene

FmnI IS04 cDNA/pcDNA3
(Plasmid #19321)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19321 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FmnI Iso4 cDNA
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4200
  • GenBank ID
    X62379
  • Entrez Gene
    Fmn1 (a.k.a. RP23-248J10.1, Fmn, ld)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FmnI IS04 cDNA/pcDNA3 was a gift from Philip Leder (Addgene plasmid # 19321 ; http://n2t.net/addgene:19321 ; RRID:Addgene_19321)

  • For your References section:

    Formin 1-isoform IV deficient cells exhibit defects in cell spreading and focal adhesion formation. Dettenhofer M, Zhou F, Leder P. PLoS ONE. 2008 . 3(6):e2497. 10.1371/journal.pone.0002497 PubMed 18560567
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