pSFFV-dNme2Cas9
(Plasmid #193190)

• Purpose: Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)
• Depositing Lab: Erik Sontheimer
• Publication: Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(18)31033-5. doi: 10.1016/j.molcel.2018.12.003.

Backbone

• Vector backbone: pSFFV
• Backbone size w/o insert (bp): 7217
• Total vector size (bp): 10663
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dNme2Cas9
• Alt name: dNme2Cas9c
• Species: N. meningitidis
• Insert Size (bp): 3246
• Mutation: H588A, D16A
• Promoter: SFFV
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS-3xHA-NLS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: N/A (unknown if destroyed)
• 3′ cloning site: N/A (unknown if destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • dNme2 SFFV backbone.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry