pLJM1-EGFP
(Plasmid #19319)

• Purpose: (Empty Backbone) 3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin
• Depositing Lab: David Sabatini
• Publication: Sancak et al Science. 2008 Jun 13. 320(5882):1496-501.

Backbone

• Vector backbone: pLJM1
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin
• Tag / Fusion Protein:
  • EGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: XL10Gold or Stbl2 cells can also be used
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (not destroyed)
• 3′ cloning site: unknown (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 199 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Information for Cloning Grade DNA (Catalog # 19319-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here