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Addgene

pJBL6090
(Plasmid #193142)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15A
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cbe ZTP Riboswitch sfGFP
  • Species
    Clostridium beijerinckii
  • Insert Size (bp)
    1000
  • Mutation
    Deleted A26 to disable ligand-binding
  • Promoter J23119

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer tcgataagcttccgatggcg
  • 3′ sequencing primer GTTTTCCGTATGTTGCATCACC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJBL6090 was a gift from Julius Lucks (Addgene plasmid # 193142 ; http://n2t.net/addgene:193142 ; RRID:Addgene_193142)

  • For your References section:

    Tuning strand displacement kinetics enables programmable ZTP riboswitch dynamic range in vivo. Bushhouse DZ, Lucks JB. Nucleic Acids Res. 2023 Mar 2:gkad110. doi: 10.1093/nar/gkad110. 10.1093/nar/gkad110 PubMed 36864761
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