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pLVX-CAPN7-C290S-mCherry
(Plasmid #193047)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193047 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAPN7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2439
  • Mutation
    C290S, siRNA resistant to: GCACCCAUACCUUUACAUU
  • GenBank ID
    NM_014296
  • Entrez Gene
    CAPN7 (a.k.a. CALPAIN7, PALBH)
  • Promoter Tight TRE
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-CAPN7-C290S-mCherry was a gift from Wesley Sundquist (Addgene plasmid # 193047 ; http://n2t.net/addgene:193047 ; RRID:Addgene_193047)

  • For your References section:

    The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission. Paine EL, Skalicky JJ, Whitby FG, Mackay DR, Ullman KS, Hill CP, Sundquist WI. Elife. 2023 Sep 29;12:e84515. doi: 10.7554/eLife.84515. 10.7554/eLife.84515 PubMed 37772788
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