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Addgene

pCAG-CAPN7-OSF
(Plasmid #193041)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193041 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAPN7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2439
  • GenBank ID
    NM_014296
  • Entrez Gene
    CAPN7 (a.k.a. CALPAIN7, PALBH)
  • Promoter Chicken Beta-actin
  • Tag / Fusion Protein
    • OSF (One-Strep-FLAG) (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-CAPN7-OSF was a gift from Wesley Sundquist (Addgene plasmid # 193041 ; http://n2t.net/addgene:193041 ; RRID:Addgene_193041)

  • For your References section:

    The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission. Paine EL, Skalicky JJ, Whitby FG, Mackay DR, Ullman KS, Hill CP, Sundquist WI. Elife. 2023 Sep 29;12:e84515. doi: 10.7554/eLife.84515. 10.7554/eLife.84515 PubMed 37772788
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