pCA528 IST1 L328D, L355A (1-366)
(Plasmid
#193040)
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PurposeBacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 193040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCA528
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameIST1
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Alt nameKIAA0174
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SpeciesH. sapiens (human)
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Insert Size (bp)1098
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MutationL328D, L355A
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GenBank IDNM_001270975.2
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Entrez GeneIST1 (a.k.a. CHMP8, OLC1)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis-Sumo (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 For
- 3′ sequencing primer T7 Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCA528 IST1 L328D, L355A (1-366) was a gift from Wesley Sundquist (Addgene plasmid # 193040 ; http://n2t.net/addgene:193040 ; RRID:Addgene_193040) -
For your References section:
The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission. Paine EL, Skalicky JJ, Whitby FG, Mackay DR, Ullman KS, Hill CP, Sundquist WI. Elife. 2023 Sep 29;12:e84515. doi: 10.7554/eLife.84515. 10.7554/eLife.84515 PubMed 37772788
