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Addgene

AAV-PGK-Chst3
(Plasmid #193024)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193024 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene plasmid #24593
  • Backbone manufacturer
    Patrick Aebischer
  • Total vector size (bp) 6054
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Chst3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1512
  • Entrez Gene
    Chst3 (a.k.a. C6ST, C6ST-1, GST-0)
  • Promoter PGK
  • Tags / Fusion Proteins
    • myc-tag (C terminal on insert)
    • 3xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site AfeI (destroyed during cloning)
  • 5′ sequencing primer Not applicable
  • 3′ sequencing primer Not applicable
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-PGK-Chst3 was a gift from Bart Nieuwenhuis (Addgene plasmid # 193024 ; http://n2t.net/addgene:193024 ; RRID:Addgene_193024)

  • For your References section:

    Chondroitin 6-sulphate is required for neuroplasticity and memory in ageing. Yang S, Gigout S, Molinaro A, Naito-Matsui Y, Hilton S, Foscarin S, Nieuwenhuis B, Tan CL, Verhaagen J, Pizzorusso T, Saksida LM, Bussey TM, Kitagawa H, Kwok JCF, Fawcett JW. Mol Psychiatry. 2021 Jul 16. pii: 10.1038/s41380-021-01208-9. doi: 10.1038/s41380-021-01208-9. 10.1038/s41380-021-01208-9 PubMed 34272488
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