AAV-PGK-eGFP
(Plasmid
#193023)
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PurposeAAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (PGK) promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 193023 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneAAV-mPGK-eGFP (beta-globin intron)
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Backbone manufacturerBart Nieuwenhuis
- Total vector size (bp) 4871
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Modifications to backboneAAV-PGK-eGFP was made by removing the beta-globin intron upstream of the eGFP sequence in the vector backbone
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeGFP
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Insert Size (bp)720
- Promoter PGK
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Not applicable
- 3′ sequencing primer Not applicable (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-PGK-eGFP was a gift from Bart Nieuwenhuis (Addgene plasmid # 193023 ; http://n2t.net/addgene:193023 ; RRID:Addgene_193023) -
For your References section:
Improving adeno-associated viral (AAV) vector-mediated transgene expression in retinal ganglion cells: comparison of five promoters. Nieuwenhuis B, Laperrousaz E, Tribble JR, Verhaagen J, Fawcett JW, Martin KR, Williams PA, Osborne A. Gene Ther. 2023 Jan 13. doi: 10.1038/s41434-022-00380-z. 10.1038/s41434-022-00380-z PubMed 36635457
