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Addgene

AAV-CMV-eGFP
(Plasmid #193022)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193022 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene plasmid #67634
  • Backbone manufacturer
    Connie Cepko
  • Total vector size (bp) 6392
  • Modifications to backbone
    AAV-CMV-eGFP was made by removing the beta-globin intron upstream of the eGFP sequence in Addgene plasmid #67634
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • Insert Size (bp)
    720
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-CMV-eGFP was a gift from Bart Nieuwenhuis (Addgene plasmid # 193022 ; http://n2t.net/addgene:193022 ; RRID:Addgene_193022)

  • For your References section:

    Improving adeno-associated viral (AAV) vector-mediated transgene expression in retinal ganglion cells: comparison of five promoters. Nieuwenhuis B, Laperrousaz E, Tribble JR, Verhaagen J, Fawcett JW, Martin KR, Williams PA, Osborne A. Gene Ther. 2023 Jan 13. doi: 10.1038/s41434-022-00380-z. 10.1038/s41434-022-00380-z PubMed 36635457
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